Hemichordates

From: Subject: Hemichordates Date: Fri, 18 Sep 2009 13:16:59 -0400 MIME-Version: 1.0 Content-Type: multipart/related; type="text/html"; boundary="----=_NextPart_000_0019_01CA3862.4D7AE2D0" X-MimeOLE: Produced By Microsoft MimeOLE V6.00.2900.5512 This is a multi-part message in MIME format. ------=_NextPart_000_0019_01CA3862.4D7AE2D0 Content-Type: text/html; charset="utf-8" Content-Transfer-Encoding: quoted-printable Content-Location: http://www.lowelab.org/Lowe_Lab/Hemichordates.html =EF=BB=BF Hemichordates=

Hemichordates

2009

The phylum Hemichordata is composed = of two=20 extant classes: the solitary burrowing marine worms or enteropneusts, = and the=20 pterobranchs, which are small, colonial, sessile organisms. Bateson = originally=20 placed this group within the chordates due to many of the adult = morphological=20 affinities of the two groups, and it was not until much later that they = were=20 reclassified into their own phylum. Current molecular phylogenies place = them as=20 the sister group of the echinoderms. Despite the close relationship of = these two=20 groups, their respective adult body plans are highly divergent, and even = gross=20 axial morphological comparisons between the groups are problematic. =

For any consideration of deuterostome = evolution=20 hemichordates are a critical component: their key phylogenetic placement = and=20 proposed morphological affinities with the chordates make them an = intriguing but=20 poorly described group. Our work, and that of other labs, is beginning = to=20 generate a substantial amount of gene expression data for conserved=20 developmental regulatory genes, allowing a more comprehensive = phylogenetic=20 sampling of developmental data within the deuterostome lineage. These = results=20 are beginning to provide critical insights into early deuterostome = evolution,=20 early nervous system evolution, and the evolutionary origins of = chordates. Only=20 by a comprehensive understanding of the body plans of all three of the=20 deuterostome phyla can we hope to reconstruct the early evolutionary = history of=20 this major metazoan lineage, and understand the transitions that led to = the=20 unique and unusual body plans of this group.

We work on = Saccoglossus=20 kowalevskii found up and down the eastern seaboard of the USA. It = lives=20 intertidally in sandy, protected bays and is a direct-developing = species =20 with abbreviated development and no true larval stage. Our fieldwork is = carried=20 out at the Marine Biological Laboratory at = Woods=20 Hole, MA in September; the main reproductive period for this worm. Our = work=20 focuses on the development of the adult body plan and nervous system = patterning.=20

Hemichordate=20 research

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Questions

Body plan=20 comparisons: How can we compare the body plan of = hemichordates and=20 chordates? What insights do these data give into the origin of chordates = ?

Approach: Large scale EST and genome = sequencing.=20 Broad expression study of developmental regulatory genes in each = species.=20 Functional analysis of selected genes

Chosen=20 species: The enteropneust hemichordate=20 Saccoglossus kowalevskii.

Photo by=20 Michael LaBarbara

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spans=3DgetElementsByTagAndClassName(parentNode,"span",key);spans.forEach= (function(node)=0A= {var contentType=3Dreplacements[key][0];var = newContent=3Dreplacements[key][1];if(contentType=3D=3D"text")=0A= {removeAllChildNodes(node);node.appendChild(document.createTextNode(newCo= ntent));}=0A= else if(contentType=3D=3D"html")=0A= {node.innerHTML=3DnewContent;}});});}=0A= Element.addMethods({selectFirst:function(element,tag_name){var = elements=3D$(element).select(tag_name);return(elements.length>0)?$(elemen= ts[0]):null;},appendChildrenFromMarkup:function(element,markup)=0A= {element=3D$(element);try=0A= {detectBrowser();if(windowsInternetExplorer&&browserVersion=3D=3D7)=0A= {element.insertAdjacentHTML("beforeEnd",markup);}=0A= else=0A= {var = dummyDiv=3D$(document.createElement('div'));dummyDiv.innerHTML=3Dmarkup;d= ummyDiv.immediateDescendants().each(function(child){element.appendChild(c= hild);});}}=0A= catch(e)=0A= {}=0A= return element;},ensureHasLayoutForIE:function(element)=0A= {element=3D$(element);detectBrowser();if(windowsInternetExplorer&&browser= Version<7)=0A= {if(!element.currentStyle.hasLayout)=0A= {element.style.zoom=3D1;}}},setFilter:function(element,filterName,filterP= arams)=0A= {element=3D$(element);var regex=3Dnew = RegExp(filterName+'\$[^\$]*\\);','gi');element.style.filter=3Delement.s= tyle.filter.replace(regex,'')+=0A= filterName+'('+filterParams+'); ';return = element;},killFilter:function(element,filterName)=0A= {element=3D$(element);var regex=3Dnew = RegExp(filterName+'\$[^\$]*\\);','gi');element.style.filter=3Delement.s= tyle.filter.replace(regex,'');return element;}});function = IWURL(urlString)=0A= {try=0A= {if((arguments.length=3D=3D0)||(arguments.length=3D=3D1&&(urlString=3D=3D= ""||urlString=3D=3Dnull)))=0A= {this.p_initWithParts(null,null,null,null,null);}=0A= else if(arguments.length=3D=3D1)=0A= {urlString.replace("file://localhost/","file:///");var = urlParts=3DurlString.match(/^([A-Z]+):\/\/([^/]*)((\/[^?#]*)(\?([^#]*))?(= #(.*))?)?/i);if(urlParts)=0A= {this.p_initWithParts(urlParts[1],urlParts[2],urlParts[4]||"/",urlParts[6= ]||null,urlParts[8]||null);}=0A= else=0A= {urlParts=3DurlString.match(/^([^?#]*)(\?([^#]*))?(#(.*))?/);if(urlParts)=0A= {this.p_initWithParts(null,null,urlParts[1],urlParts[3]||null,urlParts[5]= ||null);}=0A= else=0A= {}}}}=0A= catch(e)=0A= {print("Exception Parsing URL:"+e);}}=0A= Object.extend(IWURL,{p_normalizePathComponents:function(components)=0A= {var index=3D0;while(index0)=0A= {var = previousComponent=3Dcomponents[index-1];if(previousComponent=3D=3D"/")=0A= {components.splice(index,1);}=0A= else if(previousComponent!=3D"..")=0A= {components.splice(index-1,2);index-=3D1;}=0A= else=0A= {index+=3D1;}}=0A= else=0A= {index+=3D1;}}=0A= else=0A= {index+=3D1;}}=0A= return = components;}});Object.extend(IWURL.prototype,{p_initWithParts:function(in= Protocol,inAuthority,inPath,inQuery,inFragment)=0A= {this.mProtocol=3DinProtocol;this.mAuthority=3DinAuthority;this.mQuery=3D= inQuery;this.mFragment=3DinFragment;this.mPathComponents=3Dnull;if(inPath= )=0A= {this.mPathComponents=3DinPath.split('/');if(this.mPathComponents[0]=3D=3D= "")=0A= this.mPathComponents[0]=3D'/';for(var = i=3D0;i0)&&(this.mP= rotocol=3D=3Dbase.mProtocol)&&(this.mAuthority=3D=3Dbase.mAuthority))=0A= {var commonAncestorIndex=3D0;for(var = index=3D0;indexcommonAncestorIndex;--up)=0A= {relativePath.push("..");}=0A= for(var = down=3DcommonAncestorIndex+1;down0)=0A= {var element=3Delements.shift();var = children=3D$(element).select('.'+effectClass);if(children.length>0)=0A= {elements.minusArray(children);effectQueue=3DeffectQueue.concat(this.p_ge= nerateQueueForEffectClass(effect,effectClass,children));}=0A= effectQueue.push({element:element,effect:effect});}=0A= return effectQueue;}=0A= var allStyleSheetsLoaded=3Dfalse;var = timeStyleSheetsAppearedInDOM=3Dundefined;IWEffectRegistry.prototype.p_all= StyleSheetsLoaded=3Dfunction()=0A= {if(isCamino||isFirefox)=0A= {if(timeStyleSheetsAppearedInDOM!=3D=3Dundefined)=0A= {duration=3D(new = Date().getTime())-timeStyleSheetsAppearedInDOM;if(duration>100)=0A= {allStyleSheetsLoaded=3Dtrue;timeStyleSheetsAppearedInDOM=3Dundefined;}}=0A= else if(!allStyleSheetsLoaded)=0A= {for(var i=3D0,sheetCount=3Ddocument.styleSheets.length;i0&= &duration<100&&readyToApplyEffects)=0A= {var = queueEntry=3Dqueue.shift();if(queueEntry&&queueEntry.effect&&queueEntry.e= lement)=0A= {queueEntry.effect.applyToElement(queueEntry.element);}=0A= duration=3D(new Date().getTime())-startTime;}=0A= if(queue.length>0)=0A= {setTimeout(this.p_applyEffectsFromQueue.bind(this,queue),0);}=0A= else=0A= {performPostEffectsFixups();}}=0A= function IWChildOffset(child,parent,positionedOnly)=0A= {var l=3D0;var t=3D0;if(parent)=0A= {var current=3Dchild;while(current&¤t!=3Dparent)=0A= {if(!positionedOnly||(current.style.position=3D=3D"absolute")||(current.s= tyle.position=3D=3D"relative"))=0A= {l+=3Dcurrent.offsetLeft;t+=3Dcurrent.offsetTop;}=0A= current=3Dcurrent.parentNode;}}=0A= return new IWPoint(l,t);}=0A= function IWImageExtents(ancestor,images,left,top,right,bottom)=0A= {var unionedBounds=3Dnew IWRect(left,top,right-left,bottom-top);for(var = e=3D0;e0)&&(imageClippedBounds.size.height>0))=0A= {if((unionedBounds.size.width>0)&&(unionedBounds.size.height>0))=0A= {unionedBounds=3DunionedBounds.union(imageClippedBounds);}=0A= else=0A= {unionedBounds=3DimageClippedBounds.clone();}}}=0A= var = extents=3D{left:unionedBounds.origin.x,top:unionedBounds.origin.y,right:u= nionedBounds.origin.x+unionedBounds.size.width,bottom:unionedBounds.origi= n.y+unionedBounds.size.height};return extents;}=0A= function IWImageChildren(element)=0A= {var allImgs=3Delement.getElementsByTagName("img");var inlineRegex=3Dnew = RegExp('\\b'+'inline-block'+'\\b');var badgeRegex=3Dnew = RegExp('\\b'+'badge-overlay'+'\\b');var imgs=3Dnew Array();for(var = e=3D0;e0);var divBounds=3Dnew = IWRect(-leftOffset,-topOffset,frameSize.width,frameSize.height).round();i= f(fillBackground)=0A= {context.fillStyle=3D'rgba(0,0,0,1)';divBounds.fill(context);}=0A= for(var k=3D0;k=3D4)=0A= {context.shadowColor=3D"rgba("+parseInt(components[1],16)+", = "+parseInt(components[2],16)+", "+parseInt(components[3],16)+", = "+self.mOpacity+")";}=0A= else=0A= {components=3Dself.mColor.match(/rgb$([0-9\.]+),[ ]*([0-9\.]+),[ = ]*([0-9\.]+)$/);if(components&&components.length>=3D4)=0A= {context.shadowColor=3D"rgba("+components[1]+", "+components[2]+", = "+components[3]+", "+self.mOpacity+")";}=0A= else=0A= {iWLog("not using shadow alpha, failed to match = "+self.mColor);usingShadowAlpha=3Dfalse;}}}=0A= if(usingShadowAlpha=3D=3Dfalse)=0A= {context.globalAlpha*=3Dself.mOpacity;context.shadowColor=3Dself.mColor;}=0A= context.shadowBlur=3Dself.mBlurRadius;context.shadowOffsetX=3Dself.mOffse= t.x;context.shadowOffsetY=3Dself.mOffset.y;context.drawImage(workingCanva= s,0,0);context.restore();if(usingShadowAlpha=3D=3Dfalse)=0A= {drawImageUnshadowed=3Dself.mOpacity<1.0;}=0A= else=0A= {drawImageUnshadowed=3Dfalse;}}=0A= if(drawImageUnshadowed)=0A= {context.drawImage(workingCanvas,0,0);}=0A= if(fillBackground)=0A= {divBounds.clear(context);context.save();context.globalAlpha=3Dopacity;co= ntext.rect(divBounds.origin.x,divBounds.origin.y,divBounds.size.width,div= Bounds.size.height);context.clip();for(var = k=3D0;k0)=0A= {for(var j=3D0;j=3Dthis.imgCount)=0A= {allImagesLoaded=3Dtrue;for(var = k=3D0;allImagesLoaded&&k';clippingDivPost=3D'';thumbRect.origi= n.x-=3Dleft;thumbRect.origin.y-=3Dtop;}=0A= var markup=3D'

';markup+=3DclippingDivPre;markup+=3DimageS= treamEntry.thumbnailMarkupForRect(thumbRect);markup+=3DclippingDivPost;ma= rkup+=3Dthis.p_imageMarkup(imageSize,2);markup+=3D'

';return = markup;}=0A= IWStrokeParts.prototype.applyToElement=3Dfunction(div)=0A= {if(div!=3Dnull)=0A= {var size=3Dnew = IWSize(div.offsetWidth,div.offsetHeight);$(div).appendChildrenFromMarkup(= this.p_imageMarkup(size));if(div.className.indexOf("flowDefining")<0)=0A= {if(div.style.position!=3D'absolute')=0A= {var divRect=3Dnew IWRect(0,0,div.offsetWidth,div.offsetHeight);var = unionRect=3DIWZeroRect();var = layoutRects=3Dthis.p_imageLayout(size);layoutRects.each(function(r)=0A= {unionRect=3DunionRect.union(r);});var = padding=3DdivRect.paddingToRect(unionRect);var = marginLeft=3DElement.getStyle(div,"marginLeft");marginLeft=3DmarginLeft?(= toPixelsAtElement(div,marginLeft,false)):0;var = marginTop=3DElement.getStyle(div,"marginTop");marginTop=3DmarginTop?(toPi= xelsAtElement(div,marginTop,true)):0;var = marginRight=3DElement.getStyle(div,"marginRight");marginRight=3DmarginRig= ht?(toPixelsAtElement(div,marginRight,false)):0;var = marginBottom=3DElement.getStyle(div,"marginBottom");marginBottom=3Dmargin= Bottom?(toPixelsAtElement(div,marginBottom,true)):0;detectBrowser();if(wi= ndowsInternetExplorer)=0A= {div.style.marginLeft=3Dpx(Math.max(0,padding.left-1)+marginLeft);div.sty= le.marginTop=3Dpx(Math.max(0,padding.top-1)+marginTop);div.style.marginRi= ght=3Dpx(Math.max(0,padding.right-1)+marginRight);div.style.marginBottom=3D= px(Math.max(0,padding.bottom-1)+marginBottom);if(browserVersion=3D=3D7)=0A= {updateListOfIE7FloatsFix(div);}}=0A= else=0A= {div.style.marginLeft=3Dpx(padding.left+marginLeft);div.style.marginTop=3D= px(padding.top+marginTop);div.style.marginRight=3Dpx(padding.right+margin= Right);div.style.marginBottom=3Dpx(padding.bottom+marginBottom);}}}}}=0A= IWStrokeParts.prototype.strokeExtra=3Dfunction(imageSize)=0A= {if(!imageSize)=0A= {imageSize=3Dthis.mMaxImageSize;}=0A= rect=3Dnew IWRect(IWZeroPoint(),imageSize);var = layout=3Dthis.p_imageLayout(rect.size);var = unionRect=3DIWZeroRect();layout.each(function(r)=0A= {unionRect=3DunionRect.union(r);});return rect.paddingToRect(unionRect);}=0A= function IWStroke(strokeURL,strokeRect,maxImageSize)=0A= {this.mStrokeURL=3DstrokeURL;this.mStrokeRect=3DstrokeRect;this.mMaxImage= Size=3DmaxImageSize;}=0A= IWStroke.prototype.p_strokeRect=3Dfunction(imageSize)=0A= {var hScale=3DimageSize.width/this.mMaxImageSize.width;var = vScale=3DimageSize.height/this.mMaxImageSize.height;var = strokeRect=3Dthis.mStrokeRect.scale(hScale,vScale,true);return = strokeRect;}=0A= IWStroke.prototype.p_imageMarkup=3Dfunction(imageSize,zIndex)=0A= {var style=3Dthis.p_strokeRect(imageSize).position();if(zIndex)=0A= {style+=3D'z-index: '+zIndex+';';}=0A= return imgMarkup(this.mStrokeURL,style);}=0A= IWStroke.prototype.markupForImageStreamEntry=3Dfunction(imageStreamEntry,= imageSize)=0A= {var rect=3Dnew IWRect(0,0,imageSize.width,imageSize.height);var = markup=3D'

';markup+=3DimageStreamEntry.thumbnailMarku= pForRect(rect);markup+=3Dthis.p_imageMarkup(imageSize,2);markup+=3D'';return markup;}=0A= IWStroke.prototype.applyToElement=3Dfunction(div)=0A= {if(div!=3Dnull)=0A= {var size=3Dnew = IWSize(div.offsetWidth,div.offsetHeight);$(div).appendChildrenFromMarkup(= this.p_imageMarkup(size));if(div.className.indexOf("flowDefining")<0)=0A= {if(div.style.position!=3D'absolute')=0A= {var divRect=3Dnew IWRect(0,0,div.offsetWidth,div.offsetHeight);var = padding=3DdivRect.paddingToRect(this.mStrokeRect);var = marginLeft=3DElement.getStyle(div,"marginLeft");marginLeft=3DmarginLeft?(= toPixelsAtElement(div,marginLeft,false)):0;var = marginTop=3DElement.getStyle(div,"marginTop");marginTop=3DmarginTop?(toPi= xelsAtElement(div,marginTop,true)):0;var = marginRight=3DElement.getStyle(div,"marginRight");marginRight=3DmarginRig= ht?(toPixelsAtElement(div,marginRight,false)):0;var = marginBottom=3DElement.getStyle(div,"marginBottom");marginBottom=3Dmargin= Bottom?(toPixelsAtElement(div,marginBottom,true)):0;div.style.marginLeft=3D= px(padding.left+marginLeft);div.style.marginTop=3Dpx(padding.top+marginTo= p);div.style.marginRight=3Dpx(padding.right+marginRight);div.style.margin= Bottom=3Dpx(padding.bottom+marginBottom);detectBrowser();if(windowsIntern= etExplorer&&browserVersion=3D=3D7)=0A= {updateListOfIE7FloatsFix(div);}}}}}=0A= IWStroke.prototype.strokeExtra=3Dfunction(imageSize)=0A= {if(imageSize=3D=3D=3Dundefined)=0A= {imageSize=3Dthis.mMaxImageSize;}=0A= var imageRect=3Dnew IWRect(IWZeroPoint(),imageSize);return = imageRect.paddingToRect(this.p_strokeRect(imageSize));}=0A= function IWEmptyStroke()=0A= {}=0A= IWEmptyStroke.prototype.markupForImageStreamEntry=3Dfunction(imageStreamE= ntry,imageSize)=0A= {var rect=3Dnew IWRect(0,0,imageSize.width,imageSize.height);var = markup=3D'

';markup+=3DimageStreamEntry.thumbnailMarku= pForRect(rect);markup+=3D'

';return markup;}=0A= IWEmptyStroke.prototype.applyToElement=3Dfunction(div)=0A= {}=0A= IWEmptyStroke.prototype.strokeExtra=3Dfunction()=0A= {return new IWPadding(0,0,0,0);}=0A= var kSFRFrameTopLeft=3D0;var kSFRFrameTop=3D1;var = kSFRFrameTopRight=3D2;var kSFRFrameRight=3D3;var = kSFRFrameBottomRight=3D4;var kSFRFrameBottom=3D5;var = kSFRFrameBottomLeft=3D6;var kSFRFrameLeft=3D7;var kSFRFrameClip=3D0;var = kSFRFrameStretchEvenly=3D1;var kSFRFrameStretchToFit=3D2;function = IWPhotoFrame(images,maskImages,tilingMode,assetScale,leftInset,topInset,r= ightInset,bottomInset,unscaledLeftWidth,unscaledTopHeight,unscaledRightWi= dth,unscaledBottomHeight,leftTileHeight,topTileWidth,rightTileHeight,bott= omTileWidth,adornmentURL,adornmentPosition,adornmentSize,minimumAssetScal= e)=0A= {this.mImages=3Dimages;this.mMaskImages=3DmaskImages;this.mTilingMode=3Dt= ilingMode;this.mLeftInset=3DleftInset;this.mTopInset=3DtopInset;this.mRig= htInset=3DrightInset;this.mBottomInset=3DbottomInset;this.mUnscaledLeftWi= dth=3DunscaledLeftWidth;this.mUnscaledTopHeight=3DunscaledTopHeight;this.= mUnscaledRightWidth=3DunscaledRightWidth;this.mUnscaledBottomHeight=3Duns= caledBottomHeight;this.mLeftTileHeight=3DleftTileHeight;this.mTopTileWidt= h=3DtopTileWidth;this.mRightTileHeight=3DrightTileHeight;this.mBottomTile= Width=3DbottomTileWidth;this.mAdornmentURL=3DadornmentURL;this.mAdornment= Position=3DadornmentPosition;this.mAdornmentSize=3DadornmentSize;this.mMi= nimumAssetScale=3DminimumAssetScale;this.setAssetScale(assetScale);}=0A= IWPhotoFrame.prototype.setAssetScale=3Dfunction(assetScale)=0A= {assetScale=3DMath.min(assetScale,1.0);assetScale=3DMath.max(this.mMinimu= mAssetScale,assetScale);this.mAssetScale=3DassetScale;this.mLeftWidth=3Dt= his.scaledValue(this.mUnscaledLeftWidth);this.mTopHeight=3Dthis.scaledVal= ue(this.mUnscaledTopHeight);this.mRightWidth=3Dthis.scaledValue(this.mUns= caledRightWidth);this.mBottomHeight=3Dthis.scaledValue(this.mUnscaledBott= omHeight);}=0A= IWPhotoFrame.prototype.scaledValue=3Dfunction(valueToScale)=0A= {return Math.ceil(valueToScale*this.mAssetScale);}=0A= IWPhotoFrame.prototype.markupForImageStreamEntry=3Dfunction(imageStreamEn= try,size)=0A= {var oldAssetScale=3Dthis.mAssetScale;var = maximumScale=3Dthis.maximumAssetScaleForImageSize(size);if((maximumScale<= oldAssetScale)&&(maximumScale>=3Dthis.mMinimumAssetScale))=0A= {this.setAssetScale(maximumScale);}=0A= var coverageRect=3Dthis.coverageRect(new = IWRect(0,0,size.width,size.height));var imageRect=3Dnew = IWRect(-coverageRect.origin.x,-coverageRect.origin.y,size.width,size.heig= ht);coverageRect=3DcoverageRect.offsetToOrigin();var markup=3D'

';markup+=3DimageStreamEntry.thumbn= ailMarkupForRect(imageRect);if(maximumScale>=3Dthis.mMinimumAssetScale)=0A= {if(this.mImages!=3Dnull)=0A= {markup+=3Dthis.p_buildFrame(this.mImages,coverageRect.size,2);}=0A= if(this.mAdornmentURL!=3Dnull)=0A= {markup+=3Dthis.p_adornmentMarkupForRect(imageRect,2);}=0A= if(this.mMaskImages)=0A= {}}=0A= markup+=3D'

';if(oldAssetScale!=3Dthis.mAssetScale)this.setAssetScal= e(oldAssetScale);return markup;}=0A= IWPhotoFrame.prototype.strokeExtra=3Dfunction()=0A= {var adornmentExtraTopMargin=3D0;if(this.mAdornmentURL)=0A= {adornmentExtraTopMargin=3DMath.max(0,(this.scaledValue(this.mAdornmentSi= ze.height)-this.mTopHeight)/2.0-this.mAdornmentPosition.y);}=0A= return new = IWPadding(this.mLeftWidth-this.scaledValue(this.mLeftInset),this.mTopHeig= ht-this.scaledValue(this.mTopInset)+adornmentExtraTopMargin,this.mRightWi= dth-this.scaledValue(this.mRightInset),this.mBottomHeight-this.scaledValu= e(this.mBottomInset));}=0A= IWPhotoFrame.prototype.applyToElement=3Dfunction(div)=0A= {if(div!=3Dnull)=0A= {var markup=3D'';var divRect=3Dnew = IWRect(0,0,div.offsetWidth,div.offsetHeight);if((divRect.size.width>=3D(t= his.scaledValue(this.mLeftInset)+this.scaledValue(this.mRightInset)))&&(d= ivRect.size.height>=3D(this.scaledValue(this.mTopInset)+this.scaledValue(= this.mTopInset))))=0A= {if(this.mImages!=3Dnull)=0A= {var coverageRect=3Dthis.coverageRect(divRect);var containerRect=3Dnew = IWRect(coverageRect.origin.x,coverageRect.origin.y,0,0);markup+=3D'

';markup+=3Dthis.p_buildFrame(this= .mImages,coverageRect.size);markup+=3D'

';}=0A= if(this.mAdornmentURL!=3Dnull)=0A= {markup+=3Dthis.p_adornmentMarkupForRect(divRect);}}=0A= $(div).appendChildrenFromMarkup(markup);if(div.className.indexOf("flowDef= ining")<0)=0A= {if(div.style.position!=3D'absolute')=0A= {var frameExtra=3Dthis.strokeExtra();var = marginLeft=3DElement.getStyle(div,"marginLeft");marginLeft=3DmarginLeft?(= toPixelsAtElement(div,marginLeft,false)):0;var = marginTop=3DElement.getStyle(div,"marginTop");marginTop=3DmarginTop?(toPi= xelsAtElement(div,marginTop,true)):0;var = marginRight=3DElement.getStyle(div,"marginRight");marginRight=3DmarginRig= ht?(toPixelsAtElement(div,marginRight,false)):0;var = marginBottom=3DElement.getStyle(div,"marginBottom");marginBottom=3Dmargin= Bottom?(toPixelsAtElement(div,marginBottom,true)):0;div.style.marginLeft=3D= px(frameExtra.left+marginLeft);div.style.marginTop=3Dpx(frameExtra.top+ma= rginTop);div.style.marginRight=3Dpx(frameExtra.right+marginRight);div.sty= le.marginBottom=3Dpx(frameExtra.bottom+marginBottom);detectBrowser();if(w= indowsInternetExplorer&&browserVersion=3D=3D7)=0A= {updateListOfIE7FloatsFix(div);}}}}}=0A= IWPhotoFrame.prototype.maximumAssetScaleForImageSize=3Dfunction(in_imgSiz= e)=0A= {var = maxScale=3D1;if((in_imgSize.width>this.mLeftInset+this.mRightInset)&&(in_= imgSize.height>this.mTopInset+this.mBottomInset))=0A= {maxScale=3D1;}=0A= else = if((in_imgSize.width=3Din_img= Size.width)&&((this.mLeftInset+this.mRightInset)>0))=0A= {var = leftChunkRatio=3DMath.floor(this.mLeftInset/(this.mLeftInset+this.mRightI= nset)*in_imgSize.width)/this.mLeftInset;var = rightChunkRatio=3DMath.floor(this.mRightInset/(this.mLeftInset+this.mRigh= tInset)*in_imgSize.width)/this.mRightInset;leftChunkRatio-=3DfloatEpsilon= ;rightChunkRatio-=3DfloatEpsilon;maxWidthScale=3DMath.max(leftChunkRatio,= rightChunkRatio);if(in_imgSize.width<(Math.ceil(this.mLeftInset*maxWidthS= cale)+Math.ceil(this.mRightInset*maxWidthScale)))=0A= {maxWidthScale=3DMath.min(leftChunkRatio,rightChunkRatio);}=0A= if((maxWidthScale=3Din_imgSize.h= eight)&&((this.mTopInset+this.mBottomInset)>0))=0A= {var = topChunkRatio=3DMath.floor(this.mTopInset/(this.mTopInset+this.mBottomIns= et)*in_imgSize.height)/this.mTopInset;var = bottomChunkRatio=3DMath.floor(this.mBottomInset/(this.mTopInset+this.mBot= tomInset)*in_imgSize.height)/this.mBottomInset;topChunkRatio-=3DfloatEpsi= lon;bottomChunkRatio-=3DfloatEpsilon;maxHeightScale=3DMath.max(topChunkRa= tio,bottomChunkRatio);if(in_imgSize.height<(Math.ceil(this.mTopInset*maxH= eightScale)+Math.ceil(this.mBottomInset*maxHeightScale)))=0A= {maxHeightScale=3DMath.min(topChunkRatio,bottomChunkRatio);}=0A= if((maxHeightScale20)=0A= {IWAssert(function(){return true},"Please remove this assert and the = surrouding block.");iWLog("Too many frame image tiles are getting = generated. Performance may be affected.");}=0A= if(tilingMode=3D=3DkSFRFrameStretchEvenly)=0A= {offset=3D(end-start)/maxTiles;if(vertical)=0A= {imageRect.size.height=3Doffset;}=0A= else=0A= {imageRect.size.width=3Doffset;}}=0A= else if(tilingMode=3D=3DkSFRFrameClip)=0A= {markup+=3D'

";}}}=0A= return markup;}=0A= ------=_NextPart_000_0019_01CA3862.4D7AE2D0 Content-Type: application/octet-stream Content-Transfer-Encoding: quoted-printable Content-Location: http://www.lowelab.org/Lowe_Lab/Hemichordates_files/Hemichordates.js // Created by iWeb 2.0.4 local-build-20090502=0A= =0A= function writeMovie1()=0A= {detectBrowser();if(windowsInternetExplorer)=0A= {document.write('

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